rabbit polyclonal twist antibody  (Abcam)

Journal: Theranostics

Article Title: Vitamin D binding protein (VDBP) hijacks twist1 to inhibit vasculogenic mimicry in hepatocellular carcinoma

doi: 10.7150/thno.90322

Figure Lengend Snippet: Materials

Article Snippet: Rabbit polyclonal anti-Twist1 , , Proteintech , CAT#25465-1-AP , RRID: AB_2880093.

Techniques: Luciferase, Reporter Gene Assay, Silver Staining, Binding Assay, Molecular Weight, In Situ, Membrane, Magnetic Beads, Transfection, Modification, H&E Stain, Staining, cDNA Synthesis, SYBR Green Assay, Bicinchoninic Acid Protein Assay, In Vivo, Plasmid Preparation, CRISPR, Knock-Out, Sequencing, Software, Imaging

Journal: iScience

Article Title: AKT2-mediated nuclear deformation leads to genome instability during epithelial-mesenchymal transition

doi: 10.1016/j.isci.2023.106992

Figure Lengend Snippet:

Article Snippet: Rabbit polyclonal anti-Twist , Santa Cruz Biotechnology , Cat#sc-15393.

Techniques: Recombinant, Staining, Plasmid Preparation, Software, Imaging

Journal: iScience

Article Title: LRRC15 expression indicates high level of stemness regulated by TWIST1 in mesenchymal stem cells

doi: 10.1016/j.isci.2023.106946

Figure Lengend Snippet: The movie shows time-lapse imaging of the MSCs treated with siTWIST1. This movie file shows 24 h observation in fast forward.

Article Snippet: Rabbit Polyclonal anti - TWIST1 antibody , Cell Signaling , Cat# 46702; RRID: AB_2799308.

Techniques:

Journal: iScience

Article Title: LRRC15 expression indicates high level of stemness regulated by TWIST1 in mesenchymal stem cells

doi: 10.1016/j.isci.2023.106946

Figure Lengend Snippet: Senescence-associated changes of human and mice MSCs (A) Morphological changes of the MSCs by continuous cultures. Senescent cells showed β-galactosidase expressions. Scale bar = 500 μm. (B) TWIST1/Twist1 expressions were suppressed in the senescent MSCs. Asterisk means significant difference detected at p < 0.05 (N = 3, biological replicates). (C) Gene expressions of stemness associated genes in the MSCs at each passage number. Asterisks mean significant difference were detected at p < 0.05 between control (N = 3, biological replicates).

Article Snippet: Rabbit Polyclonal anti - TWIST1 antibody , Cell Signaling , Cat# 46702; RRID: AB_2799308.

Techniques: Control

Journal: iScience

Article Title: LRRC15 expression indicates high level of stemness regulated by TWIST1 in mesenchymal stem cells

doi: 10.1016/j.isci.2023.106946

Figure Lengend Snippet: Suppression of TWIST1/Twist1 affected stem cell characteristics of the MSCs (A) Morphological changes of the MSCs by siTWIST1 treatment. Scale bar = 500 μm. (B) Relative cell numbers counted at 72 h after siRNA treatment in the mouse primary MSCs (mMSCs). Asterisks mean significant difference were detected at p < 0.05 between control (N = 3, biological replicates). (C) Relative cell numbers counted 72 h after siRNA treatment in human MSCs (hMSCs). Asterisks mean significant difference were detected at p < 0.05 between control (N = 3, biological replicates). (D) Western blot analysis of a cell cycle suppression and senescence marker P16INK4A in the siTWIST1-treated MSCs. (E) Heatmap comparisons of stemness-associated gene expressions between the scrambled RNA-transfected control and the siTWIST1/Twist1-treated MSCs both in human and mice. Numbers on the color scale are relative values to the no-treatment control as 1.0 and the values represent the average of three independent samples.

Article Snippet: Rabbit Polyclonal anti - TWIST1 antibody , Cell Signaling , Cat# 46702; RRID: AB_2799308.

Techniques: Control, Western Blot, Marker, Transfection

Journal: iScience

Article Title: LRRC15 expression indicates high level of stemness regulated by TWIST1 in mesenchymal stem cells

doi: 10.1016/j.isci.2023.106946

Figure Lengend Snippet: Overexpression of TWIST1/Twist1 by a Sendai virus vector (SeV) in mouse and human MSCs (A) SeV resulted in high-efficient and stable gene introduction both in human and mice MSCs. Scale bar = 500 μm. (B) Efficiency of the SeV-TWIST1 transfection was observed by FACS analysis based on the GFP expression that was co-expressed with TWIST1. (C) TWIST1-overexpresing MSCs showed higher proliferation activity than the control cells transfected with SeV-GFP. Asterisks mean significant difference were detected at p < 0.05 (N = 3, biological replicates). (D) Western blot analysis of a proliferating cell marker PCNA in the TWIST1-overexpressing MSCs. (E) Gene expressions of the stemness-associated transcription factors and therapeutic cytokines in the SeV-GFP (control) and SeV-TWIST1-treated MSCs. Numbers on the color scale are relative values. Expression values used for the heatmap were the average obtained in three independent samples.

Article Snippet: Rabbit Polyclonal anti - TWIST1 antibody , Cell Signaling , Cat# 46702; RRID: AB_2799308.

Techniques: Over Expression, Virus, Plasmid Preparation, Transfection, Expressing, Activity Assay, Control, Western Blot, Marker

Journal: iScience

Article Title: LRRC15 expression indicates high level of stemness regulated by TWIST1 in mesenchymal stem cells

doi: 10.1016/j.isci.2023.106946

Figure Lengend Snippet: LRRC15 correlated with the expression of TWIST1 in human and mouse MSCs (A) Heatmap analysis with two independent samples as replicate. Common trends were observed between same experimental group. (B) Gene ontology (GO) enrichment analysis of differentially upregulated genes in the TWIST1 overexpressing human bone marrow MSCs. (C) Scatterplot analysis of differentially expressed genes in the human bone marrow MSCs overexpressing TWIST1 or GFP. (D) Expression changes of LRRC15 gene under different gene expression levels of TWIST1 in human and mice MSCs. Y axis show relative expression level to non-treatment control cells. Asterisk means significant difference detected at p < 0.05 (N = 3, biological replicates). (E) Increased expression of LRRC15 protein in the TWIST1-overexpressing human MSCs.

Article Snippet: Rabbit Polyclonal anti - TWIST1 antibody , Cell Signaling , Cat# 46702; RRID: AB_2799308.

Techniques: Expressing, Gene Expression, Control

Journal: iScience

Article Title: LRRC15 expression indicates high level of stemness regulated by TWIST1 in mesenchymal stem cells

doi: 10.1016/j.isci.2023.106946

Figure Lengend Snippet: Characteristics of the LRRC15-positive cells in the mice bone marrow tissues (A) FACS analysis for the Lrrc15-expressing population in the CD11b/ − CD45 - /Ter119 - /7-AAD - fractions. (B) Comparison of Twist1 gene expression level between the Lrrc15 - and Lrrc15 + populations. Asterisk means significant difference were detected at p < 0.05 (N = 6 from different animals). (C) Colony formation properties of the FACS-sorted cells from CD11b/ − CD45 - /Ter119 - /7-AAD - /Lrrc15 - (upper) or CD11b − /CD45 - /Ter119 - /7-AAD - /Lrrc15 + (lower) fractions. (D) Flow cytometry analysis for the CD11b − /CD45 - /Ter119 - /7-AAD - /Lrrc15 + cells with the MSC markers in mice. Gray histograms show control cells treated with isotype antibodies, and pink histograms show samples treated with each antibody. (E) FACS sorting of the MSCs using anti-CD105 and anti-LRRC15 antibodies. (F) Comparing gene expressions of Twist1, stemness-associated transcription factors, and therapeutic cytokines in the CD11b − /CD45 - /Ter119 - /CD105 + /Lrrc15 - and CD11b − /CD45 - /Ter119 - /CD105 + /Lrrc15 + cells. Asterisks mean significant differences detected at p < 0.05 (N = 3, independent experiment). (G) Engrafted EGFP + cells were detected by FACS analysis. Numbers in the figures are mean values with S.D. from three independent animals per each group.

Article Snippet: Rabbit Polyclonal anti - TWIST1 antibody , Cell Signaling , Cat# 46702; RRID: AB_2799308.

Techniques: Expressing, Comparison, Gene Expression, Flow Cytometry, Control

Journal: iScience

Article Title: LRRC15 expression indicates high level of stemness regulated by TWIST1 in mesenchymal stem cells

doi: 10.1016/j.isci.2023.106946

Figure Lengend Snippet: Characteristics of the LRRC15-positive cells in the human bone marrow tissues (A) FACS analysis showing the LRRC15 + population in the CD31 - /CD45 - /Ter119 - /7-AAD - and human MSC marker CD29 + fractions. (B) Comparing gene expressions of TWIST1 , other stemness-associated transcription factors, and therapeutic cytokines between the CD31 - /CD45 - /Ter119 - /CD29 + /LRRC15 - and the CD31 - /CD45 - /Ter119 - /CD29 + /LRRC15 + MSCs. (C) Relative gene expression levels of the signature genes in the CD31 - /CD45 - /Ter119 - /CD29 + /LRRC15 + cells collected from primary cultures of 5 different donors. Heatmap shows relative expression to the CD31 - /CD45 - /Ter119 - /CD29 + /LRRC15 - cells in the primary culture of same donors each. (D) Decrease of the LRRC15 + population by in vitro culture. (E) Ratios of the LRRC15 + MSCs at primary and passage 3. Asterisk means significant difference detected at p < 0.05 (N = 5 from different donors).

Article Snippet: Rabbit Polyclonal anti - TWIST1 antibody , Cell Signaling , Cat# 46702; RRID: AB_2799308.

Techniques: Marker, Gene Expression, Expressing, In Vitro

Journal: iScience

Article Title: LRRC15 expression indicates high level of stemness regulated by TWIST1 in mesenchymal stem cells

doi: 10.1016/j.isci.2023.106946

Figure Lengend Snippet:

Article Snippet: Rabbit Polyclonal anti - TWIST1 antibody , Cell Signaling , Cat# 46702; RRID: AB_2799308.

Techniques: Software